Mankind is facing the sixth mass extinction in the Earth’s history. Scientific knowledge on the impact of human action on biodiversity, and on possible measures to counteract species loss, has considerably increased during the last decades. However, this has not contributed to stop or even slow down species extinction. In general, implementation of possible approaches remains challenging owing to political, economic, and/or societal constraints. This session aims at showcasing best practice examples of successful implementation of biodiversity knowledge in administration, companies, and stakeholder groups, thereby mainstreaming biodiversity in different sectors. Possible action is required on all scales including international, national, regional, and local levels. Moreover, indigenous knowledge along with grass-root initiatives may play a pivotal role to improve the valuation of biological diversity. Apart from agriculture, forestry, and fishery, urban and industrial environments should explicitly be taken into account, where human impact is particularly pronounced and numerous secondary effects on biodiversity are present – yet new potential for species conservation and support may arise in transformation. The compilation of best practice for mainstreaming biodiversity in this session may exemplify how scientific knowledge can contribute to improved species conservation. It further highlights that implementation may already be an important aspect when planning scientific projects.